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The sedimentation toet niay be set up immediately, or, at the latest, m the course of the next flvo hours If the sedimentation is set up immediately, the pipet may be used agom without being cleaned be- forehand otherwise it may be used only after thorough cleaning dry mg and renewed moistening with citrate solution For Betting up the reaction, the tube is moistened with a sm a l l drop of glycerm at its lower end Almost the entire length of rubber tubmg about 4 cm.
More than 00 mm. Ejajnplc One ml of CoCb solution prepared aa desenbod above requires From this stock solution the working solution is prepared by diluting 1 0 ml with water to ml in a volumetno flask.
The working solution may also be prepared from the stock eolation described m I by diluting 1 08 ml to ml nith distilled water Procedure IScven Waaeennann tubes are placed in a metal rack 05 mm.
In certain pathologic conditions changes occur which lead to flocculation of scrum proteins In a number of dilutions. It is important to use only the purest grade of NotCOj, c.
The reaction may also bo performed as follows Reageuta 1 1 1 Q NoCl and 2. PrcparaUon This tube can easily be made from a capillary of con slant djameter Melting point tubes or damaged 0 1 ml pipets are suitable.
With the aid of paper graduated m millimeters a mark is made on the glass at the mm pomt Then the tube la carefully heated m the center over a small flame As soon as the glass begins to melt it 18 bent to form a U with two unequal sidee 6 mm difference , the shorter ode bearing the mark fig 6 Fncedun Rubber tubing is attached to the short aim of the U On a wrnall watch glass contalmng a spot of Liquold powder to prevent clotting a few drops of blood from the finger or the ear lobe are mixed quickly The blood IS sucked up through the long side of the U with the aid of the rubber tubmg until ih exactly reaches the mark.
With the aid of the cell volume hematocrit it is possible to find the corrected values for the sedimentation rate, usmg the chart fig. The tubes ore shaken im NER.
The upper part of the chamber pipet a calibrated for 1 ml In divisions of 0 01 or 0 02 ml The adjoining wider part of tho pipot camos marks ot 1.
After each breath the f unn el is closed and the plasma spread out along the walls of the funnel by turning and tilting In this manner the complete saturation of the plasma with COt approximately 40 mm Hg is accomplished.
The mercury column in the rcadmg capillary must remam mtact The dilute lactic acid with nhich the plasma will react is placed m the fillmg cup of the appamtus.
The rest of the procedure and the calculation are earned out as before. The liberated gases aro adjusted to a known volume The pressure pi of this volume of gas m the chamber is read on a mercury manometer Then the gases are oithor expelled from the chamber or absorbed by suitable reagents and the pressure pi IS read at the some volume os before absorption The partial pressure P of the gas at a given volume equals pi -- ps mm Hg.
From this difference the gas volume at OXl and mm, Hg can be calculated. Corroctions for vapor pressure and capillary attraction can be omitted slnco they are the same for both readings, provided that the temponiture remains constant this is gcaerally the case during the short time mtorval of the determination The barometric presBuro will also remam constant dutmg this penod.
The outer rubber tubmg is filled mth mercury with the aid of a Kma. The bulb of the leveling appiaratus has a volume of ml and a connected through 1 meter of vacuum tubing boiled m vaseline m vacuum to a Y-shaped jomt with 2 stopcocks.
Tbo stopcock a at the upper end of the Y jomt Bcrves to let out any posaible air bubbles which might have entered the tube while fillmg it with mercury The Bide arm is connected through rubber tubmg to the levelmg bulb A stopcock with a 3 mm bore connects the vertical arm to the man omoter and the chamber through c screw-clamped rubber tubmg.
The entire apparatus with tlie open monomotor is mounted on a board This board is attached m vertical poeitlon to the edge of a table near a water tap A hole is cut m the board to permit easy rotation of the extraction chamber A water motor or any motor with rpm con be used for shaking The chamber is shaken by means of a metal rod connected to an occontno wheel of the motor on one Hide and a clamp on the water jacket on the other For cleaning the mercury is removed and the apparatus is filled with cleaning solution then washed thoroughly with water and dried with acetone Finally air la sucked through to remove all the acetone.
This can bo achieved by regulating the position of the levelmg bulb and by closing the stopcock b The stop- cock grease as descnbed on page 28 is used but the stopcocks should not be removed and greased too often- The stopcocks should bo cleaned by wipfag them mth clean Imtfreo towels.
If a leak a present, oir nil! To tho dilute solution drops of capryllo alcohol arc added and tho mixture a well shaken before uso Procedure Dran-ing of the blood separation of the plasma and Its saturation with all colar air ore performed os descnbed for the x olu metric analj'sis.
The apparatus. The plasma is saturated with air of 6. The pressure pi of the eKtmcted gases is read at a volume of 0. After food intake the CX j value rises and the alkali reserve drops, beginning approximately one hour after the meal and la st ing for sev eral hours.
In cases of mcreased ventilation of the lung a decrease in alkali reserv e IB found e. By rawmg tho levcimg bulb the manometer reservoir is filled partly mth mercury to establish a high pressure m tho apparatus.
This blue-green solution turns deep brown when oxygen is added Preparation of the blood sample The blood a taken with a dry or parafinaled syringe and transferred to a test tube contanmig a few Fla H Apparatos for Lceptof mceota free from gas.
The difference in mm Hg multiplied by the factor indicated m table results m the oxviren content m volume per cent.
We fotmd that it IS not necessary to apply a correction factor as no absorption liquid IS mtroduced mto the chamber The CO content is obtomed by subtracting 1.
The stopcock of the pipet is closed. If no mercury pump is available the blood gas apparatus can be used as follows The pipet IS connected with rubber tubmg to the glass tube above stopcock a and with stopcock b closed the levelmg bulb is lowered, drawing mercury from a reservoir mto the pipet.
After mixmg the blood gases are liberated by ahakmg for three minutes and the pressure ts read at a volume of 0.
By taismg the levelmg bulb the liquid level is brought to the height of the stopcock capillary to expel all air from the chamber During this process no blood must enter the stopcock capillary, sinoe the octyl alcohol, floating on the surface of the blood prevents the blood from leavmg the chamber Then 0 13 ml of feme cyanide solution plus excess femeyamde to account for the stopcock bore is mtroduced Into the apparatus.
The volume of the bore is usually marked by an arrow on the filling funnel. After the stopcock has been closed and sealed with mercury the chamber is evacuated by lou'enng the mar cury to the 60 mb maik and the chamber ls shakon under vacuum for three mmutoa.
The excess of salt solution is remoyed by suction with the stopcock open to the air The mercury is lowered to the 50 mb marie and the blood-salt solution mixture is saturated with air by shaking for three mmutes.
Then the oxygen is determined os described above. The blood gases are bberated by the addition U 0 07 ml of feme cyamdeHSaponin solution plus the volume of the stopcock bore the COj is absorbed with 0 1 ml of sodium hydroxide, and the oxygen with 0.
Procedure The hemoglobm is determined simultaneously according to Sahh and by detenmnmg the total oxygen capacity If the sample b weD standardized, one division of the hemometer scale corresponds to OlOgm of hemoglobm Otherwise a correction factor must be used.
Example The sample shows a total oxygen capacity of 19 09 voL per cent. This is also the case b states of blood dilution luch as occur m nephntis with hydremia.
In a normal person the circulatmg bemoglobm is satumted with oxygen m the lungs, so that b the arterial blood about 95 por cent of the amotmt of bemoglobb corresponding to the total capacity is saturated iMth oxygen Ihe arterial oxygen saturation can bo lowered by physical labor and drop below the normal range i e 92 per cent or leas.
In nervous persons with accelerated superficial respiration a lack of oxygen m the artenal blood has been observed, correspondmg to a saturation of 90 — 80 per cent and less.
In these persons no change of air occurs b the deeper respiratory passages the partial oxygen pressure b the alveolar air ts decreased and the hemogiobta is mcomplctely saturated with oxygen.
The same is fotmd m some decompensated heart diseases; carbon monoxide poisonbg and phosgene polsoamg are some of the toxic states that lead to decreased arterial oxygen saturation.
In moderate cases of phosgene polsonmg the bek of oxygen will not be severe dunng rest, but fa considcrnblo during physical work.
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